ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (5): 985-992.doi: 10.11843/j.issn.0366-6964.2016.05.016

Previous Articles     Next Articles

Effects of CK2 Inhibitor on Biological Behaviour of DF-1 Cells with High Expression and Suppression Expression of Chicken PrPC

WAN Xue-rui,YANG Run-xia,WANG Chuan,LIU Gui-lin,WU Run*   

  1. (College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China)
  • Received:2015-09-10 Online:2016-05-23 Published:2016-05-23

Abstract:

In order to detect the effect of CK2 on DF-1 cells proliferation,adhesion,invasion and apoptosis based on the higher and lower ChPrPC expression and its relationship,DF-1 cells with higher expression of chicken PrPC (DF-1-PrP),DF-1 cells with suppression expression of chicken PrPC (DF-1-SiRNA-3) and DF-1 cells were used as cell models,after they were treated with 0,25,50,100 nmol•L-1 mitoxantrone,adhesion assay,transwell assay,MTT assay,flow cytometric assay and RT-PCR analyses were used to detect cell adhesion,invasion,proliferation,apoptosis and transcription of PRNP mRNA,respectively.The data showed that the expression of PRNP mRNA of DF-1-PrP,DF-1-SiRNA-3 and DF-1 cells were all decreased with the increasing of mitoxantrone concentration,and adhesion,invasion and proliferation ability were reduced,but apoptosis rate were increased.Furthermore,under the same mitoxantrone concentration,adhesion,invasion and proliferation ability of DF-1-PrP cells was higher than DF-1 cells,apoptosis rate was lower,but which of DF-1-SiRNA-3 cells was opposite.Results indicated that higher expression of ChPrPC promoted DF-1 cells adhesion,invasion and proliferation and inhibited apoptosis,but suppression expression of ChPrPC was opposite,CK2 plays an important role in those processes.Data of this study lay the foundation for further clarify the molecular mechanism of ChPrPC physiological function.

CLC Number: